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. 2022 Apr 4;8:157. doi: 10.1038/s41420-022-00963-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a ChIP peaks of MYCN in the EIF4EBP1 promoter region in Kelly NB cell line. b Scheme of the EIF4EBP1 promoter reporter highlighting the three E-boxes corresponding to MYCN binding sites. c HEK-293-T cells were transfected with the wildtype EIF4EBP1 promoter firefly luciferase construct and with the indicated amounts of MYCN expressing plasmid (pMYCN). A Renilla Luciferase vector was used as an internal control. d MYCN and 4EBP1 protein expression were monitored in cell lysates from c by immunoblot analyses using the indicated antibodies. e HEK-293-T were transfected with wildtype or different E-box mutants EIF4EBP1 promoter firefly luciferase constructs with or without a MYCN expressing plasmid (pMYCN). A Renilla Luciferase vector was used as an internal control. Statistics were determined using Student’s t test or Mann–Whitney U-test. Exact p-values are presented. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.