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. 2022 Apr 4;8:157. doi: 10.1038/s41420-022-00963-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Relative MYCN and EIF4EBP1 mRNA levels upon siRNA-mediated knockdown of MYCN in the MYCN-amplified IMR-32 (a) and Kelly (b) cell lines, as measured by qRT-PCR. c, d SHEP-TR-MYCN cells were treated with doxycycline (1 µg/ml) for the indicated times; EIF4EBP1 mRNA levels were determined by qRT-PCR (c) and levels of MYCN and 4EBP1 proteins were monitored by immunoblot using the indicated antibodies (d). The different 4EBP1 bands correspond to different phosphorylated forms of 4EBP1. e, f Expression levels of MYCN (e) or EIF4EBP1 (f) mRNA in SH-SY5Y cells treated with RA for the indicated times (Takeda’s dataset, n = 2 for each time point; [60]). Statistics were calculated for each time point compared to the control 0 h time point. g Expression levels of EIF4EBP1 mRNA plotted against expression levels of MYCN mRNA in SH-SY5Y cells treated with RA (Takeda’s dataset, n = 2 for each time point; [60]). h Relative EIF4EBP1 mRNA expression in healthy control tissues (ganglia, n = 9) and NB tumors (n = 26) of a TH-MYCN transgenic mouse model of NB (Balamuth’s dataset; [47]). Data were retrieved from the R2: Genomics Analysis and Visualization Platform. Statistics were determined using Student’s t test or Mann–Whitney U-test. Exact p-values are presented. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.