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. 2022 Apr 4;8:162. doi: 10.1038/s41420-022-00972-z

Fig. 7. Confirmation of the involvement of apoptotic pathway in GDF15-induced muscle atrophy.

Fig. 7

C2C12 myotubes were treated with MC38-GDF15-OE cell medium, exosomes or C26-GDF15-SH cell medium, exosomes respectively. A Analysis of the protein level of MHC, GDF15, Bcl-2, Bax, Cleaved caspase-3, Atrogin1 in C2C12 myotubes treated with MC38-GDF15-OE cell medium (n = 3, *p < 0.05, **p < 0.01; t test). B Analysis of the protein level of MHC, GDF15, Bcl-2, Bax, Cleaved caspase-3, Atrogin1 in C2C12 myotubes treated with MC38-GDF15-OE cells derived exosomes (n = 3, *p < 0.05, **p < 0.01; t test). C Analysis of the protein level of MHC, GDF15, Bcl-2, Bax, Cleaved caspase-3, Atrogin1 in C2C12 myotubes treated with C26-GDF15-SH cell medium (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001; t test). D Analysis of the protein level of MHC, GDF15, Bcl-2, Bax, Cleaved caspase-3, Atrogin1 in C2C12 myotubes treated with C26-GDF15-SH cells derived exosomes (n = 3, *p < 0.05, **p < 0.01; t test). Data presented are the mean ± SEM.