Fig. 4. DAP3 orchestrates splicing regulatory networks by modulating alternative splicing of splicing factors.

a Functional protein association networks of genes that are involved in mRNA processing and have their splicing modulated by DAP3 were identified in Metascape⁴⁵(http://metascape.org). b Semiquantitative RT-PCR analyses of splicing changes of 10 representative splicing factors RBM6, RBM4, FMR1, PRPF4B, SNRPB, TIAL1, NSRP1, AKAP17A, TIA1, and HNRNPH1 upon DAP3 depletion in EC109 and KYSE180 cells. c, d Semiquantitative RT-PCR analyses of splicing changes of RBM6, HNRNPH1, AKAP17A, and TIA1 upon inhibition of NMD in EC109 cells. Cells were treated with CHX or vehicle control (DMSO) for 6 h (c) or transfected shUPF1 or the scramble control (Scr) for 48 h (d). b–d Data are represented as mean ± s.d. of n = 3 biologically independent samples. Statistical significance is determined by unpaired, two-tailed Student’s t-test (*p < 0.05, **p < 0.01). Exact p-values and source data are provided in Source Data file.