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. 2022 Apr 4;8:17. doi: 10.1038/s41522-022-00278-z

Fig. 1. Extracellular RNA maintains biofilm structural scaffolds.

Fig. 1

a Staphylococcus aureus MR10 24-h biofilms were treated with the indicated enzymes for 30 min at 37 °C or left untreated (Control). b MR10 biofilms were formed in the absence (Control) or presence of the indicated enzymes for 24 h at 37 °C. c The numbers of viable cells in the 24-h biofilms formed in the absence (Control) or presence of the indicated enzymes were counted as colony-forming units (CFUs) per 1 mL. d The growth of MR10 was measured in BHIN with or without RNase A (80 μg/mL) in a 96-well plate under static conditions. The absorbance of each well at 600 nm was measured with an incubation reader (Scinics Corporation, Tokyo, Japan). e MR10 24-h biofilms were treated or not with RNase A. RNase inhibitor was added (+). In addition, the RNase inhibitor was denatured at 105 °C for 5 min and then added (+D). Biofilm biomasses are presented as the mean and standard deviation (error bar) of three independent experiments. **P < 0.01. N.S. not significance.