Table 1.
Bacterial strains and plasmids used in this study.
| Name | Descriptiona | Source and reference |
|---|---|---|
| Staphylococcus aureus strains | ||
| MR10 | A clinical isolate of MRSA from the Jikei hospital | 26 |
| MR10 ΔicaA | The ΔicaA isogenic strain of MR10 | This study |
| RN4220 | A restriction deficient derivative of 8325-4 used for sub-cloning host | 28 |
| USA300 | A clinical isolate of MRSA | 29 |
| MR3 | A clinical isolate of MRSA from the Jikei hospital | 31 |
| MS7 | A clinical isolate of MSSA from the Jikei hospital | 31 |
| MS8 | A clinical isolate of MSSA from the Jikei hospital | 31 |
| MS9 | A clinical isolate of MSSA from the Jikei hospital | 31 |
| MS12 | A clinical isolate of MSSA from the Jikei hospital | 31 |
| SH1000 | 8325-4 with functional rsbU | 30 |
| Escherichia coli strain | ||
| DH5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | BioDynamics Laboratoy (Tokyo, Japan) |
| Plasmids | ||
| pGEM-T Easy Vector | AmpR, M13ori pBR322ori, linear-T-overhand vector | Promega |
| pKOR1 | An E. coli-S. aureus shuttle vector plasmid for knockout of staphylococcal genes by allelic exchange, CmR, AmpR | 43 |
| pKOR1-icaAKO | A pKOR1-derivative plasmid for knockout of the icaA gene in MR10, CmR, AmpR | This study |
| pLC1 | An E. coli-S. aureus shuttle vector plasmid, CmR, AmpR | 48 |
| pLC1::ica | pLC1-derivative plasmid for expression of ica operon containing the ribosome-binding site of icaA, CmR, AmpR | This study |
aAmpR, ampicillin resistance; CmR, Chloramphenicol resistance.