Table 2.

The selected sequence was used as the template for RT-PCR.

Sequence name	Sequence (from 5′ to 3′)a
fragment9959_count111_len101	GAAAGCACCG TTTCCCGTCC GATCAACTGT AGTTAAGCTG GTAAGAGCCT GACCGAGTAG TGTAGTGGGT GACCATACGC GAAACTCAGG TGCTGCAATC T
fragment8400_count133_len100	TCATTTGTAT ACGACTTAGA TGTACAACGG GGTATTGTAA GCAGTAGAGT AGCCTTGTTG TTACGATCTG CTGAGATTAA GCCTTTGTTG TCTGATTTGT

^aAmong the reads obtained by RNA-seq, long sequences (>100 nt) with more than 100 counts were selected to confirm whether these RNAs were derived from BHI medium. The fragments were amplified by RT-PCR, and the products were analyzed using agarose gel electrophoresis (Fig. 3b).