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. 2022 Apr 4;13:1787. doi: 10.1038/s41467-022-29503-1

Fig. 2. PripA localizes to macropinosomes.

Fig. 2

a Schematic representation of PripA, PripA-PH, and PripA-ΔPH. b–d Time lapse images of PripA-GFP, PripA-PH-GFP, and PripA-ΔPH-GFP expressed in WT cells. The asterisks indicate newly formed macropinosomes. The arrows indicate smaller-sized vesicles labeled by PripA or PripA-ΔPH that surrounded newly formed macropinosomes. e–g TD uptake in WT cells expressing GFP-tagged PripA-PH, PripA-ΔPH, or PripA. The asterisks indicate newly formed macropinosomes containing weak TD signal. The arrows mark smaller macropinosomes labeled by PripA or PripA-ΔPH containing bright TD signal. h–j DQ-BSA degradation in WT cells expressing GFP-tagged PripA-PH, PripA-ΔPH, or PripA. The asterisks indicate newly formed macropinosomes containing minimal DQ-BSA signal. The arrows mark vesicles labeled by PripA or PripA-ΔPH containing bright DQ-BSA signal. Magnified images of the areas indicated by dashed boxes are shown on the right. Scale bar = 5 μm.