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. 2021 Dec 15;126(7):1037–1046. doi: 10.1038/s41416-021-01651-z

Fig. 5. Nuplazid inhibits ESCC growth through PAK4.

Fig. 5

a The knockdown efficiency of PAK4 by shRNA (#2, #5) in KYSE150 and KYSE450 cells was evaluated by Immunoblotting. And the level of the PAK4 downstream signal MAPK in the shPAK4 cells was detected by WB. b Cell numbers of KYSE150 and KYSE450 cells with transfected shPAK4 at 0, 24, 48 and 72 h. c Colonies were counted for KYSE150 and KYSE450 cells with transfected with shPAK4 after 7 days. d The overexpression efficiency of PAK4 in KYSE410 cells was evaluated by WB and the level of the PAK4 downstream signal MAPK1 in the KYSE410 cells with transfected PAK4 was detected by WB. e Cell numbers of KYSE410 cells with transfected PAK4 at 0, 24, 48 and 72 h. f Colonies were counted for KYSE410 cells with transfected PAK4 after 7 days. g The KYSE150 cells and KYSE450 cells were treated with 2.5 μM Nuplazid for 96 h, and the inhibition rate was calculated. All data are shown as means ± S.D. The asterisks (*, **, ***) indicate a significant decrease (p < 0.05, p < 0.01, p < 0.001, respectively).