Table 3.
List of CPP-PNAs used in this study.
| CPP-PNA | Target mRNA | Sequencec | References |
|---|---|---|---|
| KFF-acpP | E. coli acpP | KFFKFFKFFK-O_linker-ctcatactct | 19 |
| KFF-NC | Nonea | KFFKFFKFFK-O_linker-atactaacag | This study |
| KFF-bamB | E. coli bamB | KFFKFFKFFK-O_linker-catcgggtcc | This study |
| KFF-tolC | E. coli tolC | KFFKFFKFFK-O_linker-tgcattcctt | This study |
| RXR-NC | Noneb | RXRRXRRXRRXRXB-ctacttatgcd | This study |
| RXR-bamB | P. aeruginosa bamB | RXRRXRRXRRXRXB-catatcattg | This study |
| RXR-oprM | P. aeruginosa oprM | RXRRXRRXRRXRXB-tcaggcctct | This study |
CPP-PNA, cell-penetrating peptide conjugate of peptide nucleic acid; acpP, a gene for acyl carrier protein AcpP; bamB, a gene for outer membrane lipoprotein BamB; tolC, a gene for outer membrane efflux protein TolC; oprM, a gene for outer membrane protein OprM.
aNo match with E. coli MG1655 genome as antisense.
bNo match with P. aeruginosa PAO1 genome as antisense.
cUppercase and lowercase letters indicate peptide and PNA sequences, respectively, and PNA sequences are shown from the N-terminus to the C-terminus. The O_linker portion is composed of polyethylene glycol.
d X and B indicate 6-aminohexanoic acid and beta-alanine residues, respectively.