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. 2022 Apr 4;12:5609. doi: 10.1038/s41598-022-09635-6

Table 3.

List of CPP-PNAs used in this study.

CPP-PNA Target mRNA Sequencec References
KFF-acpP E. coli acpP KFFKFFKFFK-O_linker-ctcatactct 19
KFF-NC Nonea KFFKFFKFFK-O_linker-atactaacag This study
KFF-bamB E. coli bamB KFFKFFKFFK-O_linker-catcgggtcc This study
KFF-tolC E. coli tolC KFFKFFKFFK-O_linker-tgcattcctt This study
RXR-NC Noneb RXRRXRRXRRXRXB-ctacttatgcd This study
RXR-bamB P. aeruginosa bamB RXRRXRRXRRXRXB-catatcattg This study
RXR-oprM P. aeruginosa oprM RXRRXRRXRRXRXB-tcaggcctct This study

CPP-PNA, cell-penetrating peptide conjugate of peptide nucleic acid; acpP, a gene for acyl carrier protein AcpP; bamB, a gene for outer membrane lipoprotein BamB; tolC, a gene for outer membrane efflux protein TolC; oprM, a gene for outer membrane protein OprM.

aNo match with E. coli MG1655 genome as antisense.

bNo match with P. aeruginosa PAO1 genome as antisense.

cUppercase and lowercase letters indicate peptide and PNA sequences, respectively, and PNA sequences are shown from the N-terminus to the C-terminus. The O_linker portion is composed of polyethylene glycol.

d X and B indicate 6-aminohexanoic acid and beta-alanine residues, respectively.