TABLE 2.
Antibodies and their conditions used in this investigation.
| Antibody | Antigen | Clone | Dilution | Secondary antibody | Phosphorylation site | Significance | Supplier |
|---|---|---|---|---|---|---|---|
| mTOR markers | pmTOR | #2976 | 1:100 | Novolink | Ser2448 | mTORC1, mTORC2 | Cell Signaling (Boston, MA, United States) |
| pS6 | #2211 | 1:100 | Novolink | Ser235/236 | mTORC1 | Cell Signaling (Boston, MA, United States) | |
| rictor | A500-002A | 1:1000 | Vectastain | mTORC2 | Bethyl Laboratories, Inc. (Montgomery, TX, United States) | ||
| Metabolic markers | PFK | #8164 | 1:100 | Novolink | Glycolysis | Cell Signaling (Boston, MA, United States) | |
| LDHA | #3582 | 1:400 | Novolink | Glycolysis | Cell Signaling (Boston, MA, United States) | ||
| ATPB | ab14730 | 1:100 | Novolink | Oxydative phosphorylation | Abcam, Cambridge, United Kingdom | ||
| G6PDH | ab133525 | 1:100 | Novolink | Pentose phosphate pathway | Abcam, Cambridge, United Kingdom | ||
| GLS | ab156876 | 1:200 | Novolink | Glutaminolysis | Abcam, Cambridge, United Kingdom | ||
| CPT1A | ab128568 | 1:500 | Novolink | Beta oxydation of fatty acids | Abcam, Cambridge, United Kingdom | ||
| FASN | #3180 | 1:50 | Novolink | Long chain fatty acid synthesis | Cell Signaling (Boston, MA, United States) |