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. 2022 Mar 22;13:858215. doi: 10.3389/fphar.2022.858215

FIGURE 2.

FIGURE 2

μCT of femurs showed the increased trabecular number and correspondingly reduced spacing in p62 KO mice. (A) Trabecular (grey) and cortical (red) bone structure of the femur of WT and p62 KO mice. (B) Bone volume per total bone volume (BV/TV) was similar between WT and p62 KO mice and showed no effect of treatment. (C) Trabecular number (Tb.N) was significantly increased in vehicle-treated p62 KO mice compared to vehicle-treated WT mice. No effect of genotype was observed after treatment. (D) Trabecular spacing (Tb.Sp) was reduced in vehicle-treated p62 KO mice compared to WT mice. (E) Trabecular thickness (Tb.Th) showed no difference between p62 KO and WT mice nor an effect of treatment. (F) Tissue mineral density (TMD) of trabecular bone was similar between genotypes and showed no effect of treatment. (G) Cortical thickness (Ct.Th) of the femur was not influenced by the treatment and showed no difference between genotypes. (H) Cortical porosity (Ct. Po) and (I) tissue mineral density (TMD) of cortical bone were similar between genotypes and not influenced by the treatment with JWH133. Male mice were aged 3 months by the beginning of the experiment and were treated for 5 days with vehicle or JWH133. Data were analyzed by using ordinary two-way ANOVA and Bonferroni adjusted p values, *p < 0.05, **p < 0.01, ***p < 0.001. All error bars show mean ± SEM. Squares and circles represent individual data points. WT vehicle N = 7, KO vehicle N = 8, WT JWH133 N = 8, KO JWH133 N = 8.