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. 2022 Mar 22;13:858215. doi: 10.3389/fphar.2022.858215

FIGURE 9.

FIGURE 9

6-month aged WT and p62 KO mice showed an opposing effect on osteoclasts after prolonged treatment with JWH133. (A) Number of osteoblasts per bone perimeter (N.Ob/B.Pm) was comparable in vehicle- and JWH133-treated mice and showed no effect of genotype or treatment. (B) Number of osteoclasts per bone perimeter (N.Oc/B.Pm) was significantly increased in p62 KO mice (vehicle) compared to WT mice (vehicle). The treatment affected genotypes in an opposite way. (C) Osteoblast surface per bone surface (Ob.S/BS) was not altered between genotypes and was not affected by the treatment. (D) Osteoclasts per bone surface (Oc.S/BS) were weakly increased in WT mice and reduced in p62 KO mice by the treatment with JWH133 leading to a significant effect of interaction. Data were analyzed by using ordinary two-way ANOVA and Bonferroni adjusted p values, *p < 0.05, **p < 0.01, ***p < 0.001. All error bars show mean ± SEM. Squares and circles represent individual data points. WT vehicle N = 8, KO vehicle N = 8, WT JWH133 N = 8, KO JWH133 N = 8.