Figure 1.

MCT7 causes a rapid decrease of intracellular taurine level. HEK293T cells were transfected with EGFP-tagged MCT7 (○) or empty-vector (mock) (•). The transfected cells were incubated in Na⁺-free buffer (pH 7.4) at 37 °C for designed time. After the incubation, the amino acids contents were measured by LC-MS/MS and the obtained chromogram area is correlated by protein amount. The values were expressed as % of initial value. Each point represents the mean ± S.D. (n = 7). ∗∗ p < 0.01 and ∗ p < 0.05, compared with the corresponding mock-transfected cells by two-way ANOVA with Sidak's multiple comparisons test. EGFP, enhanced green fluorescent protein; MCT7, monocarboxylate transporter 7.