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. 2022 Mar 22;12:842312. doi: 10.3389/fonc.2022.842312

Figure 4.

Figure 4

The silencing of Sox15 was governed through promoter methylation within HCC cells. (A) Sox15 mRNA levels within HCC cells measured through RT-qPCR. (B, C) Sox15 promoter methylation status is measured by MSP and USP and quantification. (D) Sox15 mRNA levels within HCC cells measured by RT-qPCR post-exposure to 5-Aza. (E, F) Sox15 promoter methylation status is measured by MSP and USP and quantification post-exposure to 5-Aza. MSP, methylation specific PCR; USP, unmethylation specific PCR; *p < 0.05.