Table 3.

Cycling conditions for the primers of the bla_TEM and tetA genes.

Gene	Primary denaturation	Amplification (35 cycles)			Final extension
		Secondary denaturation	Annealing	Extension
bla TEM	94°C	94°C	54°C	72°C	72°C
	5 min	30 s	40 s	45 s	10 min
tetA	94°C	94°C	50°C	72°C	72°C
	5 min	30 s	40 s	45 s	10 min