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. 2022 Mar 22;13:861081. doi: 10.3389/fpls.2022.861081

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Copyright © 2022 Liu, Zhang, Pu, Cai, Sui, Rengel, Chen and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential appearance of flue-cured leaves (A), expression of CYP82E4 gene (B) and Fe concentrations (C) in leaves of CR60 and Y87. The plants were cultivated in an experimental field located at the Yanhe Research Farm of Yunnan Academy of Tobacco Agricultural Sciences, Yunan Province, China (N24.14, E102.30, altitude 1,680 m). For panel (A) the matured leaves were harvested and flue-cured following local agricultural practices. For panel (B), leaves at stalk position #10 (from top to bottom) of 2-month-old plants were harvested for RNA isolation. For panel (C) the 5th to 10th (from top to bottom) leaves were flue-cured and mixed for ICP-MS analysis. Three biological replicates were analyzed. Values are means ± SD (n = 3). Asterisks indicate significant differences between the CR60 and Y87 plants (*P < 0.05 and ***p < 0.001) as determined by the Student’s t-test.