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CiIFNa and mutants binds to CRFB1, CRFB2, and CRFB5. The HEK293 cells were transfected with respective plasmids (2.5 μg each). After 24 h, cell lysates were immunoprecipitated (IP) with antibody agarose resin. The immunoprecipitates and cell lysates were analyzed by immunoblotting (IB) with the anti-His, anti-Flag, anti-HA, or anti-Myc Abs. (A–C) Interaction of CiIFNa and mutants with CRFB1 (A), CRFB2 (B) and CRFB5 (C). "+"=transfected, "-"=not transfected.
