Table 1.

Information of gene primers.

Primer name	Sequence(5’–3’)	Application
CiIFNa	F: CATGCCATGG CTTGCGAATGGCTCGGTCGCTACCGT	Prokaryotic plasmid construction
	R: CGGGATCCTTAACGGCGATTGGCGATGCT
L27A	F: AGAATGATCAGCAACGAGAGCGCCAGCCTGCTGAAGGAG	Construction of mutant plasmid
	R: GGCGCTCTCGTTGCTGATCATTCTGTATCTGCCCAGCCA
E103A	F: CAGTGGAACCTGCAGACCGTGGCCCACTTCCTGACCGTGCTGAACAGACAGAGCAGCGAC R: GGCCACGGTCTGCAGGTTCCACTGCACGCTGTTCATGTG	Construction of mutant plasmid
K117A	F: AACAGACAGAGCAGCGACCTGGCCGAGTGCGTGGCTAGA R: AACAGACAGAGCAGCGACCTGGCCGAGTGCGTGGCTAGA	Construction of mutant plasmid
H165A	F: CAGATCAGAAGAGCCGTGAAGGCCCACCTGCAGAGAATG R: GGCCTTCACGGCTCTTCTGATCTGCTCCCAGGCTTGGGC	Construction of mutant plasmid
EPC-IFNa	F: ATGAAAACTCAAATGTGGACGTA R: GATAGTTTCCACCCATTTCCTTAA	qPCR
EPC-Mx	F: GGCTGGAGCAGGTGTTGGTATC R: TCCACCAGGTCCGGCTTTGTTAA	qPCR
EPC-Viperin	F: AGCGAGGCTTACGACTTCTG R: GCACCAACTCTCCCAGAAAA	qPCR
EPC-Isg15	F: CAGCCTTGAGGATGATTCCAG R: TGCCGTTGTAAATCAGTCG A	qPCR
EPC-IRF3	F: GTTTAGAGGGACAATTAACTGGACTA R: GAGGGTCCACTCTTTGAAAATG	qPCR
EPC-IRF7	F: AAAGTCTTCGTCAGCACCAGCG R: CTCTCCGAAGCACAGGTAGATGGT	qPCR
EPC-β-actin	F: CACTGTGCCCATCTACGAG R: CCATCTCCTGCTCGAAGTC	qPCR
SVCV-N-native	F: TCTGCCAAATCACCATACTCA R: CCATCTCCTGCTCGAAGTC	qPCR and PCR
SVCV-G- native	F: ATCATTCAAAGGATTGCATCAG R: CATATGGCTCTAAATGAACAGAA	qPCR and PCR
SVCV-N- plasmid	F: AACACTGCTGATGGAGAGCC R: TCTGCTCACGATTGTTCCCC	PCR
SVCV-P- plasmid	F: ACGAGGAGGGAACAAGCAAG R: GTGCAGTCTGAACTCGCTCT	PCR
SVCV-M- plasmid	F: GAGACACTGGCTACAGCTCC R: TATGTTCCGCTCACGTGCTT	PCR
SVCV-G- plasmid	F: ACACCGGAGAGAACGGAAAC R: CCAGGCTTCTCATCTCGTGG	PCR
SVCV-L- plasmid	F: CGACGAGGAGATCGGAAAGG R: TCGCTCATCACGATAGGCAC	PCR

Forward (F) and reverse (R) primers given in the table were used for sequencing the respective amplicons. Bold text (CT) indicates sequences introduced in the primer to avoid open reading frame shift. Underlined are sequences of restriction enzymes.

PCR, polymerase chain reaction; qPCR, quantitative PCR.