Fig. 5.

Cytocompatibility of pure Zn porous scaffold and dense pure Zn extracts: (a) In vitro cytotoxicity test of MC3T3-E1 cells cultured in pure Zn porous scaffolds with and without dilution; (b) Zn ion concentrations after immersion in culture medium for 24 h; (c) Laser scanning confocal microscopy images of MC3T3-E1 cells cultured in pure Zn porous scaffold extracts with and without dilution. The actin cytoskeleton were stained green and the nuclei were stained blue. The data (n = 3) are expressed as mean ± standard deviation (SD). *: P < 0.05.