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. 2022 Mar 22;12:726985. doi: 10.3389/fonc.2022.726985

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Copyright © 2022 Xu, He, Xiao, Si, Shi, Liu, Tian, Ren, Wu and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The new CAR-NK-92 (SP) cell line could be stably passaged and expanded independently of exogenous IL-2. (A) Short term proliferation of SP and NK92 cells exposed to 10 U/mL IL-2(left) and 100 U/mL IL-2 (right) supplements. (B) 5-Ethynyl-2’-deoxyuridine (EdU) assay and (C) cell cycle analysis were performed using SP cells and NK92 cells cultured with the 100 U/mL IL-2 supplement. (D) Shown is the percentage of apoptotic cells was detected by FCM after culturing NK92 and SP cells in PBS for 8 hours. The left sides of panels (B–D) show a representative sample image of the experimental results, and the right side shows the statistical results of three parallel experiments expressed as mean ± SEM (n = 3, **P < 0.01, ***P < 0.001).