Figure 6.
Visual and dynamical observation of the delivery process of secretory lysosomes of SP cells to PD-L1highMP cells. From left to right are the images from 12 sequential time points of the effect-target cell co-culture experiment. From top to bottom, the first row indicates fields of view under the green fluorescence (FITC) channel, the second row indicates fields of view under the red fluorescence (Cy5) channel, and the third row shows the overlay of the two-fluorescence signals. Dynamically changes can be observed following the co-culture of effector and target cells, and the red box indicates typical degranulation phenomenon, in which SP cells delivered secretory lysosomes labeled by Lyso-Tracker Red fluorescent probe to PD-L1highMP cells.