Fig. 3.

MDSCs induced CAF activation by upregulating FGF1 levels. (A) α-SMA immunostaining images of tumor tissues(Scale bar = 100μm). Positive area was analyzed by ImageJ(n = 3 mice). (B), (C) The TMP value of PDGF, FGF1 and TGFβ mRNA in tumor tissue(n = 5 mice). (D) qRT-PCR analyses for FGF1 in the BNL cells(n = 4). (E) qRT-PCR analyses for PDGF in the BNL cells(n = 3). (F)WB for FGF1 and PDGFB in BNL cells(n = 3). Specific n values of biological independent experiments. t – test for two groups comparisons. ANOVA for four groups comparisons and Tukey test for multiple comparisons. Asterisks indicate comparison with the control group. *p < 0.05, **p<0.01***, p < 0.001.