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. 2022 Apr 2;20:101407. doi: 10.1016/j.tranon.2022.101407

Figure 2.

Figure 2

3D brain tumor model viability assessment by AlamarBlue assay. A. Effects of three conditions (e.g., NB, NE and N+FBS) on (a) medulloblastoma (MB), (b) ependymoma (EPN), (c) glioblastoma (GBM) and (d) pilocytic astrocytoma (Ast). Each chart is from a single tumor case as marked in each chart title..Four replicates were used for each data point per condition per time point. Error bar, standard error of mean. Student's t-test, *, p <0.05; **, p < 0.01. B. Effects of ECM gel types infused in 3D scaffolds, e.g., collagen type I (3D-SF/Col) or Matrigel (3D-SF/Matri), compared to 3D scaffold only (3D-SF) cultures. (a-MB), 3D cultures in NB media are shown here, demonstrating significant growth-promoting effects by Matrigel. This effect was not significant in cultures in NE media. (b-EPN), 3D cultures in all media and matrix combination conditions are compared, showing the growth-promoting effect of Matrigel in all three media conditions. (c-GBM) and (d-Ast) show 3D cultures in NE media. Each chart is from a single tumor case as marked in each chart title. Four replicates were used for each data point per condition per time point. Error bar, standard error of mean. Student's t-test: *, p <0.05; **, p < 0.01.