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. 2022 Feb 26;298(4):101778. doi: 10.1016/j.jbc.2022.101778

Figure 2.

Figure 2

c-Abl phosphorylates γ-tubulin.A, Anti-Flag immunoprecipitates prepared from HEK293T cells transfected with the indicated plasmids were subjected to SDS-PAGE and immunoblotting using the indicated antibodies. B, Anti-γ-tubulin, anti-p-Tyr and IgG immunoprecipitates prepared from MCF-7 cells were analyzed with anti-γ-tubulin. The intensities of the bands were analyzed using ImageJ. C, Anti-γ-tubulin immunoprecipitates from MCF-7 cells or abl1/abl2 DKO cell lines were subjected to SDS-PAGE and immunoblotting with the indicated antibodies. D and E, Anti-Flag immunoprecipitates prepared from HEK293T cells transfected with the indicated plasmids were subjected to SDS-PAGE and immunoblotting using the indicated antibodies. Cells also treated with or without nilotinib (10 μM) or STI571 (10 μM) in E. The IP assays or Pull-down assays were repeated at least twice.