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. 2022 Feb 26;298(4):101778. doi: 10.1016/j.jbc.2022.101778

Figure 7.

Figure 7

c-Abl-mediated phosphorylation of γ-tubulin regulates spindle structure and mitosis.A and B, Wild-type and abl1−/−abl2−/− MCF-7 cells were stained with anti-γ-tubulin (green) and anti-PCNT (red). Microscopy of metaphase cells (A), spindle width (B, left) and Arc angle (B, right) are presented. C and D, U2OS cells stably expressing γ-tubulin-Flag and γ-tubulin(Y443F)-Flag were stained with anti-γ-tubulin (green) and anti-PCNT (red). Microscopy of metaphase cells (C), spindle width (D, left) and Arc angle (D, right) are presented. E, chromosome numbers in wild-type and abl1−/−abl2−/− MCF-7 cells (left panel), wild-type and abl1−/−abl2−/− MEFs (middle panel), and γ-tubulin-Flag/γ-tubulin(Y443F)-Flag-expressing MCF-7 cells (right).