Fig. 2.

Effects of EAE and CS on body weight. (a) Average body weights of the EAE and naïve control mice in all exposure groups are shown. The body weights of the mice were normalized to their respective weights on study day 15 and presented as percent change for the (b) naïve control mice and (c) EAE mice. Study day 15 corresponds to the first day of EAE induction (equivalent to day 0 post-immunization). Data presented as mean ± SEM. For naïve control mice, sham = blue lines with blue open circles (○), CS Low = pink lines with open squares (□), CS Med = red lines with open triangles (△), and CS High = brown lines with open diamonds (◇). For EAE mice, sham = blue lines with solid circles (●), CS Low = pink lines with solid squares (■), CS Med = red lines with solid triangles (▲), and CS High = brown lines with solid diamonds (◆). GAM analyses of the body weights of (d) naïve control mice and (e) EAE mice exposed to fresh air (sham) or three concentrations of CS (Low, Med, High) are shown. The black lines are average differences in body weight from pairwise comparison between the groups indicated in the headers. Grey bands represent 95% confidence intervals. The red dotted lines are set at zero to represent the body weight of the second group in each comparison (e.g., for CS Low vs. sham analysis, sham is set at zero). Note: Data from similarly exposed subgroups (e.g. 2-week, 4-week, and 6-week) were combined for presentation in (a), (b), and (c), while only 6-week groups were included in (d) and (e), as described in the Materials & Methods. n = 8 mice per group, except n = 7 in the 6-week CS Low, n = 10 in the 6-week CS Med, and n = 9 in the 6-week CS High groups (Supplemental Table 1). CS: cigarette smoke; EAE: experimental autoimmune encephalomyelitis; SEM: standard error of mean; GAM: generalized additive model.