Fig. 3.

Clinical score development in EAE mice exposed to various concentrations of CS. (a) Mean daily clinical scores for EAE mice are shown as mean ± SEM. Sham = blue lines with solid circles (●), CS Low = pink lines with solid squares (■), CS Med = red lines with solid triangles (▲), and CS High = brown lines with solid diamonds (◆). The insert shows the mean maximum clinical scores of the EAE mice. The color coding is the same as that for the mean clinical scores. (b) Clinical onset was analyzed by using non-parametric Kaplan–Meier estimator and log-rank tests. The shaded areas represent 95% confidence intervals for the survival probability of the respective groups. The p value indicated in the graph is the overall statistical significance among all groups and was not significant. (c) Disease progression and severity were analyzed by using a GAM. There were no differences in disease progression among any of the groups, as indicated by the non-crossing, parallel slopes. (d) The clinical score progression correlated well with weight loss over time. Sham = blue lines, CS Low = pink lines, CS Med = red lines, and CS High = brown lines. n = 8 mice per group, except n = 7 in the 6-week CS Low, n = 10 in the 6-week CS Med, and n = 9 in the 6-week CS High groups (Supplemental Table 1). CS: cigarette smoke; EAE: experimental autoimmune encephalomyelitis; SEM: standard error of mean; GAM: generalized additive model.