Fig. 4.

Disease prevalence in EAE mice exposed to various concentrations of CS. (a) Descriptive data indicating the average daily probability of disease occurrence are shown (n = 7–10). Sham = blue lines with solid circles (●), CS Low = pink lines with solid squares (■), CS Med = red lines with solid triangles (▲), and CS High = brown lines with solid diamonds (◆). (b) GAM analysis was used to estimate the probability of disease curves over time. Disease prevalence was the lowest in EAE mice exposed to the high concentration of CS. Sham = blue lines, CS Low = pink lines, CS Med = red lines, CS High = brown lines. n = 8 mice per group, except n = 7 in the 6-week CS Low, n = 10 in the 6-week CS Med, and n = 9 in the 6-week CS High groups (Supplemental Table 1). CS: cigarette smoke; EAE: experimental autoimmune encephalomyelitis; GAM: generalized additive model.