TABLE 1.

Specifics of the four exercise protocols used and corresponding measures.

Exercise Protocol	Speed	Incline	Duration	Corresponding Measure and Figures
Maximal running speed	2 min at 10 m min−1 then speed increased 1 m min−1 every 2 min	0°	To exhaustion	- Maximal running speed (Figure 1A)
Time to exhaustion	70% of individual maximal running speed	0°	To exhaustion	- Time to exhaustion (Figure 1B)
Exercise calorimetry	60% of individual maximal running speed	0°	15 min (final 5 min used for calorimetry analysis)	- VO2, TEE, RER and rates of CHO and fat oxidation (Figure 2)
Maximal running test at a 5° incline	2 min at 10 m min−1 then speed increased 1 m min−1 every 2 min	5°	To exhaustion	- Blood glucose, blood lactate and serum NEFA (Figure 3)
				- Change in tissue glycogen (Figure 4)
				- Correlation between maximal running speed and change in tissue glycogen content (Figure 5)
				- Skeletal muscle AMPK α and β2 content and AMPK, ACC and TBC1D1 signaling (Figure 7)