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. 2022 Mar 22;13:810834. doi: 10.3389/fimmu.2022.810834

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Copyright © 2022 Kundu, Shetty and Gomes-Solecki

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein expression of key biomarkers of necroptosis at 72h post infection. (A) represents the immunoblots of protein lysate (whole spleen cells) from uninfected Control (CON), nonpathogenic L. biflexa (LB) and pathogenic L. interrogans (LIC) infected groups. (B–G) represents the densitometric histograms normalized to β-actin expression control using ImageJ software. Legend: MLKL, Mixed Lineage Kinase Domain-Like protein; Receptor Interacting Serine/Threonine Kinase (RIP1 and RIP3), p, phosphorylated; MW, molecular weight; kD, kilodalton.