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. 2022 Mar 17;25(4):104098. doi: 10.1016/j.isci.2022.104098

Figure 5.

Figure 5

Infection of NPCs with HCMV strain TB40r mGFP-IE-FKBP virus results in limited expression of viral proteins IE1 and IE2 (UL122/UL123) unless Shield-1 is administered

NPCs were infected at 3 or 7 days post plate down with TB40r mGFP-IE-FKBP at an MOI of 1. One group was then supplemented with 1 μM Shield-1 daily (Shield-1 +) while the other was given vehicle (Shield-1 -).

(A–D) Expression of HCMV viral genes (UL122 (IE), UL123 (IE), UL44 (E), and UL99 (L)) within NPCs infected 3- or 7- days post plate down at 24, 48, and 72 hours post infection (hpi) as determined by qPCR. A significant upregulation of viral transcripts was observed in (Shield-1 +) conditions compared to (Shield-1 -). Stars were assigned based on level of significance as determined by one-way ANOVA with Tukey post hoc test: ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.00001.

(E) Immunocytochemical images at 24, 48, and 72 hpi from NPCs infected 3 days post plate down with HCMV-IE1/IE2-ddFKBP and probed for GFP (green), neurodevelopmental transcription factor SOX2 (red), UL123 (IE1) (purple), Hoescht (blue), and merge.

All images taken at 40X using Zeiss LSM980 and scale bar is 20 μm. qPCR data from 3 biological replicate experiments and error bars represent mean ± SEM.