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The effect of gracillin on autophagy-related proteins in A549 cells. (A) Western blot analysis of Beclin-1, P62, LC3-Iand LC3-II and A549 cells treated with gracillin (0, 0.25, 0.5, 1, 2 μmol/L) for 24 hours. The full picture is in Supplementary Figure 1 . (B) The relative protein expression levels of Beclin-1, P62, LC3-I, and LC3-II were quantified by normalization to GAPDH, compared with the control group, *P ≤ 0.05, ***P ≤ 0.001.
