FIGURE 4.

rhMG53 attenuates NM‐induced oxidative stress in human bronchial epithelial (B2B) porcine aortic endothelial (PAoEC) cells. The B2B cells and primary porcine aortic endothelial (PAoEC) cells were cultured for 24 h and treated with 10 µM (for PAoEC)/20 µM (for B2B cells) NM or BSA control for 4 h, and washed, incubated with BSA or rhMG53 (10 µg/ml) and cultured for another 20 h. Cells were stained and with DCF staining for ROS measurement. (A) Representative images for B2B with DCF staining. Scale bar, 25 µm. (B) Representative FACS analysis of B2B with DCF staining; (C) Quantification of B2B (FACS) for panel b (n = 3). (D) Quantification of PAoEC for analysis with DCF staining (FACS) (n = 3) [Colour figure can be viewed at wileyonlinelibrary.com]