FIGURE 3.

Transplanted sNPCs alter the host transcriptome at the transplantation site. (A_i) Eosin Y/Nissl staining of untreated contused spinal cord twelve weeks after injury. (A_ii and A_iv) Higher magnification image of the area laser microdissected in untreated contused spinal cords. (A_iii) Higher magnification image of the area laser microdissected in the treated contused spinal cord. The cavity area was approximated and 200 μm outside the edge of the cavity area was extracted to compare transcriptome profiles with the sNPC‐treated cord. (B) Unsupervised hierarchical clustering reveals distinct separation between host spinal cords with cell grafts and untreated spinal cords. Read counts were normalized and log transformed. Hierarchical clustering was performed using Euclidean distances and average linkage clustering method. The first three and last three columns represent their respective replicates. (C) Expression profile alterations between genes associated with regulation of differentiation/structural organization and ionic channel genes, respectively, between grafted and untreated spinal cords. The boxes show the 25th–75th percentile range, and the centre mark is the median. Whiskers show 1.5 times IQR from the 25th or 75th percentile values