FIGURE 1.

CD44 silencing inhibited HCT116‐CSCs proliferation, migration and invasion. (A) qPCR assay quantifies the knockdown efficiency of CD44 expression in HCT116‐CSCs; (B) MTT assay reveals that the proliferation of HCT116‐CSCs is significantly inhibited following CD44‐siRNA transfection relative to si‐NC transfection; (C) colony formation assays show that knockdown of CD44 expression results in a reduction in clonogenic survival of HCT116‐CSCs; (D) Transwell migration and invasion assays reveal that knockdown of CD44 expression inhibits the migration and invasion of HCT116‐CSCs compared with si‐NC transfection. *p < 0.05; **p < 0.01