Fig. 1.

SYR382141 decreases ceramide-1-phosphate levels in cells. A: WT pDFs pretreated with SYR382141 (100 nM), NVP-231 (100 nM), or DMSO (0.01%) for 30 min received mechanical trauma via asterisk pattern scratch across the plate. Cells were collected 2 h post-injury and analyzed for C1P levels via UPLC ESI-MS/MS. Values expressed as fold change to DMSO controls (∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001; n = 3, pDFs collected from three different mice; two-way ANOVA with Dunnett's multiple comparisons test). B: HUVECs were treated with SYR382141 (100 nM), NVP-231 (300 nM), or DMSO (0.01%) for 24 h. Cells were collected and analyzed for C1P levels via UPLC ESI-MS/MS. Values expressed as fold change to DMSO controls. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; n = 3; one-way ANOVA with Tukey post-hoc test).