Selected immunogenic cytotoxic T-lymphocyte epitopes incorporated in the vaccine. The epitopes were predicted using the NetCTL 1.2 server, and those with binding scores of less than 2 using the Immune Epitope Database (IDEB) consensus method were considered to be good binders with their respective HLA-class-1 allele. The antigenic epitopes were screened out using the VaxiJen v2.0 server.
| PDB ID | Epitopes | Position | Supertype (combined score) | MHC class 1 allele | Binding score | Immunogenicity | Antigenicity |
|---|---|---|---|---|---|---|---|
| 6VSB | WTAGAAAYY | 258 | A1 (3.1128) | HLA-A*01:01 | 0.165 | 0.152 | 0.6306 ± 0.1123 |
| A26 (2.0048) | HLA-B*35:01 | 1.2 | |||||
| B58 (1.2345) | HLA-A*26:01 | 0.11 | |||||
| B62 (1.3574) | HLA-A*30:02 | 0.115 | |||||
| HLA-A*68:01 | 1.185 | ||||||
| HLA-B*15:01 | 1.6 | ||||||
| FTISVTTEI | 718 | A2 (1.1808) | HLA-B*58:01 | 0.4 | 0.044 | 0.8535 ± 0.0479 | |
| A26 (1.0014) | HLA-B*51:01 | 0.7 | |||||
| B58 (0.9262) | HLA-A*02:01 | 0.8 | |||||
| HLA-A*68:02 | 0.2 | ||||||
| HLA-A*26:01 | 0.615 | ||||||
| 6VXX | RLDPPEAEV | 1002 | A2 (0.9436) | HLA-A*02:01 | 0.79 | 0.171 | 0.4496 ± 0.0859 |
| HLA-A*02:11 | 0.395 | ||||||
| HLA-A*02:16 | 0.405 | ||||||
| HLA-A*02:12 | 0.45 | ||||||
| HLA-A*02:19 | 0.45 | ||||||
| VVFLHVTYV | 1079 | A2 (1.0304) | HLA-A*02:01 | 1.2 | 0.127 | 1.5122 ± 0.1999 | |
| HLA-A*02:03 | 0.455 | ||||||
| HLA-A*02:06 | 0.575 | ||||||
| HLA-A*68:02 | 0.6 | ||||||
| HLA-A*69:01 | 0.955 | ||||||
| HLA-A*02:19 | 1.07 | ||||||
| HLA-A*02:11 | 1.19 | ||||||
| HLA-A*02:02 | 1.525 | ||||||
| HLA-A*68:23 | 1.245 | ||||||
| LLMGCVAET | 22 | A2 (1.0322) | HLA-A*02:01 | 0.7 | 0.054 | 0.4298 ± 0.1640 | |
| HLA-A*02:19 | 0.23 | ||||||
| HLA-A*02:03 | 0.585 | ||||||
| HLA-A*02:02 | 0.875 | ||||||
| HLA-A*02:12 | 1.315 | ||||||
| HLA-A*02:11 | 1.975 | ||||||
| HLA-A*02:16 | 1.83 | ||||||
| 6X2A | PYRVVVLSF | 492 | A24 (1.8786) | HLA-A*24:02 | 0.33 | 0.031 | 1.0281 ± 0.0505 |
| HLA-A*23:01 | 0.2 | ||||||
| HLA-A*24:03 | 0.615 | ||||||
| HLA-B*08:03 | 1.895 | ||||||
| IAIPTNFTI | 697 | A24 (1.0788) | HLA-B*51:01 | 0.2 | 0.185 | 0.7052 ± 0.1105 | |
| B58 (1.5865) | HLA-B*53:01 | 0.5 | |||||
| HLA-B*15:17 | 0.72 | ||||||
| HLA-B*57:01 | 0.865 | ||||||
| HLA-A*69:01 | 1.27 | ||||||
| HLA-B*58:01 | 0.3 | ||||||
| HLA-A*24:02 | 1.28 | ||||||
| 6XR8 | FVFLVLLPL | 2 | A26 (1.0076) | HLA-A*02:01 | 0.5 | 0.040 | 0.8601 ± 0.0927 |
| A2 (1.1947) | HLA-B*46:01 | 0.22 | |||||
| B8 (0.8252) | HLA-A*02:06 | 0.36 | |||||
| B62 (0.9849) | HLA-A*25:01 | 0.41 | |||||
| HLA-A*68:02 | 0.6 | ||||||
| HLA-A*02:02 | 0.945 | ||||||
| HLA-A*69:01 | 0.745 | ||||||
| HLA-B*35:03 | 0.565 | ||||||
| HLA-B*39:01 | 0.49 | ||||||
| WTFGAGAAL | 886 | A26 (0.9302) | HLA-B*07:02 | 1.5 | 0.197 | 0.4918 ± 0.1134 | |
| B62 (1.1379) | HLA-B*48:01 | 0.22 | |||||
| HLA-B*39:01 | 0.315 | ||||||
| HLA-B*15:17 | 0.225 | ||||||
| HLA-B*15:02 | 0.285 | ||||||
| HLA-A*68:23 | 0.125 | ||||||
| HLA-B*38:01 | 0.765 | ||||||
| HLA-A*68:02 | 0.7 | ||||||
| 6ZB4 | IPTNFTISV | 714 | B7 (1.5427) | HLA-B*07:02 | 0.7 | 0.172 | 0.8820 ± 0.0649 |
| HLA-B*51:01 | 0.8 | ||||||
| HLA-B*42:01 | 1.535 | ||||||
| HLA-B*83:01 | 1.57 | ||||||
| HLA-B*54:01 | 1.3 | ||||||
| 6ZGE | LPFNDGVYF | 115 | B7 (1.0427) | HLA-B*35:01 | 0.5 | 0.117 | 0.5593 ± 0.0501 |
| HLA-B*53:01 | 0.5 | ||||||
| HLA-B*15:03 | 1.2 | ||||||
| HLA-B*35:03 | 1.81 | ||||||
| HLA-B*42:01 | 1.07 | ||||||
| HLA-B*83:01 | 0.885 | ||||||
| HLA-B*51:01 | 0.9 | ||||||
| 7KDH | HVSGTNGTK | 69 | A3 (1.1575) | HLA-A*03:01 | 1.15 | 0.063 | 1.0956 ± 0.1256 |
| HLA-A*68:01 | 0.905 | ||||||
| HLA-A*11:01 | 1.6 | ||||||
| HLA-A*66:01 | 1.5 | ||||||
| HLA-A*26:03 | 1.265 | ||||||
| TLADAGFIK | 827 | A3 (1.2451) | HLA-A*03:01 | 0.62 | 0.281 | 0.5781 ± 0.0314 | |
| HLA-A*11:01 | 0.44 | ||||||
| HLA-A*68:01 | 0.86 | ||||||
| GVYFASTEK | 89 | A3 (1.4615) | HLA-A*03:01 | 0.19 | 0.090 | 0.7112 ± 0.1176 | |
| HLA-A*66:01 | 0.22 | ||||||
| HLA-A*11:01 | 0.23 | ||||||
| HLA-A*30:01 | 1.1 | ||||||
| HLA-A*68:01 | 1.135 |