Skip to main content
. Author manuscript; available in PMC: 2022 Apr 5.
Published in final edited form as: Cell Rep. 2022 Mar 8;38(10):110411. doi: 10.1016/j.celrep.2022.110411

Figure 4. BNRF1 supports late lytic cycle progression, viral DNA replication, and infectious virion production.

Figure 4.

(A) Heatmap of normalized EBV gene expression levels from RNA-seq analysis of P3HR-1 ZHT/RHT cells with control or BNRF1 sgRNA, mock induced or induced into the lytic cycle with 4-HT/NaB for 24 h, from 2 replicates.

(B) Flow cytometry analysis of plasma membrane (PM) gp350 expression in P3HR-1 ZHT/RHT cells expressing control BXLF1 or BNRF1 sgRNAs, mock induced or induced for lytic reactivation for 24 h with 4-HT/NaB.

(C) PM gp350 in P3HR-1 ZHT/RHT cells obtained as in (B) from 5 replicates. Error bars indicate mean ± SE.

(D) qRT-PCR of EBV intracellular genome copy numbers from P3HR-1 cells with BXLF1 or BNRF1 sgRNA induced by 4-HT/NaB for 24 h. Mean ± SE values from 3 replicates are shown.

(E) Transmission electron microscopy (TEM) of P3HR-1 ZHT/RHT cells with control or BNRF1 sgRNAs induced into the lytic cycle with 4-HT/NaB for 24 h. Scale bar, 500 nm.

(F) Percentage of empty capsids observed from TEM analysis of P3HR-1 ZHT/RHT cells with control versus BNRF1 sgRNA as in (E). Data are from 20 randomly chosen nuclear fields.

(G) Mean ± SEM from 3 replicates of green Raji assay analysis of infectious EBV titers from EBV+ Akata cells with BXLF1 or BNRF1 sgRNA induced by immunoglobulin G (IgG) crosslinking for 48 h from 3 replicates.

****p < 0.0001, **p < 0.01. See also Figure S4.