Table 2.
Recovery of infectious virus from qRT-PCR positive shedding samples and tissues after in- tranasal challenge of ferrets with SARS-CoV-2.
| Days post inoculation | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 3 | 5 | 7 | 9 | 14 | ||||||
| Sex | M | F | M | F | M | F | M | F | M | F |
| No | n = 10 | n = 10 | n = 8 | n = 8 | n = 6 | n = 6 | n = 4 | n = 4 | n = 2 | n = 3 |
| Nasal wash | 4/10 | 6/10 | 4/8 | 2/7 | 3/6 | 1/5 | 0/4 | 1/3 | – | – |
| Swab oral | 1/6 | 1/8 | 0/5 | 0/4 | 1/5 | 0/5 | 0/2 | 0/2 | – | – |
| Swab recta | 0/3 | 0/6 | 0/2 | 0/2 | 0/2 | 0/3 | – | – | – | – |
| No | n = 2 | n = 2 | n = 2 | n = 2 | n = 2 | n = 2 | n = 2 | n = 2 | n = 2 | n = 2 |
|---|---|---|---|---|---|---|---|---|---|---|
| Plasma EDT | – | – | – | – | – | – | – | – | – | – |
| Turbinate | 2/2 | 1/2 | 2/2 | 2/2 | 2/2 | 2/2 | 0/1 | – | – | – |
| Pharynx | 1/2 | 0/2 | 0/2 | 1/2 | 0/2 | 1/2 | 0/2 | 0/1 | – | – |
| Trachea | 0/1 | 0/2 | 0/1 | 0/2 | 0/1 | – | – | – | – | – |
| Lung | – | 0/1 | 0/1 | – | – | – | – | – | – | – |
| LYMPH RETR | 0/2 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | 0/1 | – | 0/2 | 0/1 |
| LYMPH BRON | – | – | 0/1 | – | – | – | – | – | – | – |
| Thymus | – | – | – | – | – | – | – | – | – | – |
| Heart | – | – | – | – | – | – | 0/1 | – | – | – |
| OLFAC LOBE | 0/1 | 0/2 | 0/1 | 1/1 | 0/1 | – | – | – | – | – |
| OCCIP LOBE | 0/2 | 0/2 | 0/1 | 0/2 | 0/1 | – | – | – | – | – |
| Stomach | 0/2 | 0/1 | 0/2 | 0/2 | 0/2 | 0/2 | – | – | – | – |
| Duodenum | 0/1 | 0/1 | 0/2 | 0/1 | 0/1 | 0/1 | – | – | – | – |
| Jejunum | – | – | – | – | 0/1 | – | – | – | – | – |
| Ileum | 0/1 | – | – | – | 0/1 | 0/1 | – | – | – | – |
| Colon | 0/1 | – | 0/1 | 0/1 | 0/2 | 0/1 | – | – | – | – |
| Liver | – | – | – | – | 0/1 | – | – | – | – | – |
| Kidney | – | – | – | – | 0/1 | 0/1 | – | – | – | – |
| Spleen | – | – | – | – | 0/1 | – | – | – | – | – |
| Ovary | × | – | × | – | × | – | × | – | × | – |
| Testicle | – | × | – | × | 0/1 | × | – | × | – | × |
The numerator indicates the number of samples that were positive by infectivity assay (> 92.8 TCID50/mL). The denominator indicates the number of qRT-PCR positive samples tested. × indicates not applicable.
LYMPH RETR the retropharyngeal lymph node, LYMPH BRON the bronchial lymph node, OLFAC LOBE the olfactory bulb, OCCIP LOBE the occipital lobe respectively.