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. 2022 Mar 22;13:847313. doi: 10.3389/fmicb.2022.847313

FIGURE 2.

FIGURE 2

Inactivation of SARS-CoV-2 with Dulbecco’s Modified Eagle Medium (DMEM; positive control), 66.5% ethanol (EtOH), 0.5% sodium hypochlorite (NaOCl), 1% potassium monopersulfate (KMPS), 100 ppm chlorine dioxide (ClO2), and 500 ppm ClO2 during the Wiperator assay. Inoculated carriers were subjected to 5 s of wiping with disinfectant pre-soaked wipes infused with each of the examined disinfectants. Sterile uninoculated “transfer” carriers demonstrate transfer potential of viable virus to a clean surface through a used disinfectant presoaked wipe. All carriers were neutralized in 1 ml of a pre-determined neutralizer following indicated treatments, with subsequent viability testing in Vero E6 cells. Dashed lines indicate limits of quantification using the TCID50 assay. Results represent the means of three independent experiments including three biological replicates each. Symbol *Indicates presence of viable virus in safety testing of a single biological replicate from the indicated treatment group.