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Experimental workflow. Peptides from a HeLa digest were subjected to online reverse-phase liquid chromatography (LC) before online gas-phase separation using high-field asymmetric ion mobility spectrometry (FAIMS) and mass spectrometry (MS) analysis. The MS data was searched in Proteome Discoverer for any multi-PTM peptides that were detected, the identified “hits” being potential PTM crosstalk site candidates.
