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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Nat Chem Biol. 2021 Oct 18;17(12):1262–1270. doi: 10.1038/s41589-021-00880-w

Extended Data Fig. 7. Lentiviral constructs for intact and split-engineered base editor editing in K562 cells.

Extended Data Fig. 7

(a) Lentiviral construct schematics for intact BE4 max, sgRNA-only, seBEC and seBEN+sgRNA are shown. (b) Quantification of GFPoff cells by flow cytometry for cells with EMX1-targeting sgRNA along with intact BE4max or seBE-IRES. Cells were either treated with no rapamycin or with rapamycin (25 nM) added at either day 3 or day 5 (marked with arrow) and then maintained continuously. Mean and standard deviation are noted, with individual data points shown (n = 3).