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. 2022 Mar 22;9:854007. doi: 10.3389/fvets.2022.854007

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Copyright © 2022 Du, Zhou, Zhou, Xue and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein expression and localization of MDV and REV examined by confocal laser scanning microscope (CLSM). The proteins of MDV and REV were co-localized in cytoplasm (arrowhead). The coinfected cells showed more cytopathy (CPE) (star) than single-infected cells. Samples stained with pp38 or gp90 antibodies as well as Cy3-labeled goat anti-rabbit IgG (for MDV) (red) or FITC-labeled goat anti-mouse IgG (for REV) (green) antibody in the assay. The areas of colocalization are shown in yellow. Cell nuclei (blue) were stained with DAPI. MDV, Marek's disease virus; REV, reticuloendotheliosis virus; CLSM, confocal laser scanning microscope; CPE, cytopathic effect; FITC, fluorescein isothiocyanate.