TABLE 2.
Kinetic parameters of Y530A-SntA.
| kcat (s–1) | Km (μM) | kcat/Km (M–1 s–1) | |
| 3′-AMP | 172.85 ± 9.57 | 1,379.38 ± 145.11 | 1.25E+05 |
| 2′,3′-cAMP | 277.34 ± 59.26 | 3,418.12 ± 790.15 | 8.11E+04 |
| 2′,3′-cGMP | 98.02 ± 14.99 | 3,245.54 ± 503.74 | 3.02E+04 |
| 2′,3′-cCMP | 197.89 ± 33.86 | 6,440.94 ± 1,528.91 | 3.07E+04 |
| pApA | Nd | Nd | 1.68E+03 |
| 2′,3′-cUMP | 83.75 ± 3.32 | 3,124.20 ± 292.86 | 2.68E+04 |
| bis-4NPhP | 129.66 ± 9.80 | 2,441.77 ± 211.17 | 5.31E+04 |
| c-di-AMP | Nd | Nd | 8.53E+01 |
| ATP | Nd | Nd | 8.93E+01 |
| Ap4A | Nd | Nd | 5.38E+01 |
| Ap3A | Nd | Nd | 3.74E+01 |
| 4-NPhP | Nd | Nd | 8.75E+01 |
| ADP | Nd | Nd | 2.60E+01 |
| ADP-ribose | Nd | Nd | 2.40E+01 |
| 3′,5′-cAMP | Nd | Nd | 6.29E+01 |
| CDP-choline | Nd | Nd | 6.79E+01 |
kcat and KM values were calculated from saturation curves obtained at different concentrations of substrates.
They are shown as mean values ± standard deviations of three experiments.
Catalytic efficiencies were calculated by dividing kcat/KM or, when these parameters were not available, by the procedure described in the section “Materials and Methods.”
Nd, not determined.