TABLE 3.
Kinetic parameters of Y633A-SntA.
| kcat (s–1) | Km (μM) | kcat/Km (M–1 s–1) | |
| 3′-AMP | 69.13 ± 3.50 | 2,769.37 ± 335.61 | 2.50E+04 |
| 2′,3′-cAMP | 281.98 ± 27.76 | 3,625.43 ± 184.72 | 7.78E+04 |
| 2′,3′-cGMP | 81.41 ± 21.53 | 5,190.24 ± 1,488.46 | 1.57E+04 |
| 2′,3′-cCMP | 37.13 ± 10.29 | 4,704.97 ± 1,234.16 | 7.89E+03 |
| pApA | Nd | Nd | 1.91E+03 |
| 2′,3′-cUMP | 33.90 ± 15.37 | 3,583.27 ± 2,010.97 | 9.46E+03 |
| bis-4NPhP | 69.18 ± 8.75 | 1,923.44 ± 208.29 | 3.60E+04 |
| c-di-AMP | Nd | Nd | 7.78E+01 |
| ATP | Nd | Nd | 6.18E+01 |
| Ap4A | Nd | Nd | 3.59E+01 |
| Ap3A | Nd | Nd | 4.55E+01 |
| 4-NPhP | Nd | Nd | 1.06E+02 |
| ADP | Nd | Nd | 3.69E+01 |
| ADP-ribose | Nd | Nd | 2.20E+01 |
| 3′,5′-cAMP | Nd | Nd | 4.80E+00 |
| CDP-choline | Nd | Nd | 2,65E+01 |
kcat and KM values were calculated from saturation curves obtained at different concentrations of substrates.
They are shown as mean values ± standard deviations of three experiments.
Catalytic efficiencies were calculated by dividing kcat/KM or, when these parameters were not available, by the procedure described in the section “Materials and Methods.”
Nd, not determined.