TABLE 4.
Kinetic parameters of double mutant Y530A+Y633A-SntA.
| kcat (s–1) | Km (μM) | kcat/Km (M–1 s–1) | |
| 3′-AMP | 0.025 ± 0.002 | 1,162.44 ± 84.76 | 2.16E+01 |
| 2′,3′-cAMP | 29.33 ± 4.09 | 3,183.95 ± 926.67 | 9.21E+03 |
| 2′,3′-cGMP | 17.65 ± 3.19 | 3,313.34 ± 446.14 | 5.33E+03 |
| 2′,3′-cCMP | 26.39 ± 7.21 | 4,785.63 ± 1,562.63 | 5.51E+03 |
| 2′,3′-cUMP | 30.95 ± 5.04 | 3,189.01 ± 607.81 | 9.71E+03 |
| bis-4NPhP | 65.85 ± 7.78 | 2,239.29 ± 327.54 | 2.94E+04 |
kcat and KM values were calculated from saturation curves obtained at different concentrations of substrates.
They are shown as mean values ± standard deviations of three experiments.
Catalytic efficiencies were calculated by dividing kcat/KM.