Fig. 5.

Time courses of growth (A), respiration (B), and expression of the genes related to respiratory oxygen consumption (COXI [C] and AOX [D]) in SL and ALT301 during culture under normal growth condition. At times indicated after a start of subculture, cells were withdrawn for determinations of FW (to monitor growth) and respiration (O₂ uptake rate), simultaneously. Cells were also harvested for expression analyses of the genes by real-time RT-PCR. Significant differences between SL and ALT301 are indicated with asterisks (*P < 0.05, **P < 0.01, Welch’s t-test). Values are the mean ± SE (n = 3 from three independent experiments).