Skip to main content
. 2022 Feb 4;9(10):2104317. doi: 10.1002/advs.202104317

Figure 8.

Figure 8

Suppressing peripheral CDYL activity by its antagonist reduces neuronal excitability and pain‐like behaviors. a) The RMP of DRG neurons treated with 10, 30, 100 µm UNC6261, 100 µm UNC7394 or vehicle for 24 h. One‐way ANOVA with Turkey's post‐hoc test, *p < 0.05 versus vehicle; # p < 0.05 versus UNC7394. b,c) Representative traces (b) and numbers (c) of APs induced by indicated currents. Scale bars, 100 ms and 30 mV. Two‐way ANOVA, group effect: *p < 0.05, **p < 0.01 versus vehicle; ## p < 0.01 versus UNC7394. d–f) The AP threshold (d), AHP amplitude (e), and rheobase (f) in DRG neurons. One‐way ANOVA with Turkey's post‐hoc test, *p < 0.05, **p < 0.01 versus vehicle; # p < 0.05, ## p < 0.01 versus UNC7394. g–i) The peak (g), amplitude (h), and half‐width (i) of AP in DRG neurons. One‐way ANOVA with Turkey's post‐hoc test, no statistical significance. n vehicle = 17, n UNC7394 = 18, n 10 µm UNC6261 = 17, n 30 µm UNC6261 = 19, n 100 µm UNC6261 = 18. j,k) Time course of PWT (j) and PWL (k) of mice after injection of 0.7 and 2.1 mg kg−1 UNC6261, 2.1 mg kg−1 UNC7394 or vehicle to DRG. Two‐way ANOVA with Sidak's post‐hoc test, *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle; ## p < 0.01, ### p < 0.001 versus UNC7394. l) Time course of PWT of SNI mice with injection of UNC6261, UNC7394 or vehicle. Two‐way ANOVA with Sidak's post‐hoc test, *p < 0.05, **p < 0.01 versus vehicle; # p < 0.05, ## p < 0.01 versus UNC7394. Data are the mean ± SEM.