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. Author manuscript; available in PMC: 2022 Apr 5.
Published in final edited form as: J Neurochem. 2008 Feb 1;105(5):1683–1699. doi: 10.1111/j.1471-4159.2008.05262.x

Fig. 2.

Fig. 2

Con A but not MβC blocks PMA-induced reduction of DAT cell surface levels. rDAT-LLCPK1 cells were treated with vehicle, 250 μg/mL Con A, or 5 mM MβC at 37°C prior to treatments with vehicle or 200 nM PMA for 30 min at 37°C. Cells were cooled to 4°C and subjected to surface biotinylation and lysates were chromatographed on NeutrAvidin beads. (a) Representative DAT immunoblot of column eluates. (b and c) Quantification of DAT biotinylation intensities from three independent experiments performed in duplicate. Data are presented as mean ± SE of band intensities relative to the non-treated sample (b) or relative to the non-PMA treated sample for each treatment group (c). *, p < 0.05***, p < 0.001 relative to control (ANOVA).